Protein_Domain

Part:BBa_K1830004:Design

Designed by: Mathieu Husser   Group: iGEM15_Concordia   (2015-08-06)

coh1C3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

GCA to GCT mmutation to remove PstI cut site


Source

Clostridium Thermocellum CipA protein, GenBank accession number: L08665.1 (nucleotide) / Q06851 (protein)

References

Wieczorek, A. S., & Martin, V. J. (2010). Engineering the cell surface display of cohesins for assembly of cellulosome-inspired enzyme complexes on Lactococcus lactis. Microbial cell factories, 9(1), 69.